主权项 |
1. A method of expanding cells in a detachable flow circuit configured to attach to a fixed portion of a cell expansion system, wherein the cell expansion system comprises an incubator and a plurality of controllers, the method comprising:
adding the cells to the detachable flow circuit, wherein the detachable flow circuit comprises:
a first fluid circulation path comprising a first fluid flow path having at least opposing ends, a first end of the first fluid flow path configured to fluidly associate with a first inlet port of a cell growth chamber, and a second opposing end of the first fluid flow path configured to fluidly associate with a first outlet port of the cell growth chamber, wherein a portion of the first fluid circulation path is configured to be disposably mounted to a first fluid controller of the fixed portion of the cell expansion system;a second fluid circulation path comprising a second fluid flow path having at least opposing ends, a first end of the second fluid flow path configured to fluidly associate with a second inlet of the cell growth chamber, and a second opposing end of the second fluid flow path configured to fluidly associate with a second outlet of the cell growth chamber, wherein a portion of the second fluid circulation path is configured to be disposably mounted to a second fluid controller of the fixed portion of the cell expansion system;a first fluid connector path having at least opposing ends, a first opposing end of the first fluid connector path fluidly associated with the first fluid circulation path and a second opposing end of the first fluid connector path fluidly associated with the second fluid circulation path, wherein a portion of the first fluid connector path is configured to be disposably mounted to a third fluid controller of the fixed portion of the cell expansion system; anda second fluid connector path having opposing ends, one end of the second fluid connector path fluidly associated with the first fluid flow path and a second end of the second fluid connector path fluidly associated with the second fluid flow path, wherein a portion of the second fluid connector path is configured to be disposably mounted to a fourth fluid controller of the fixed portion of the cell expansion system; and incubating the cells to produce an expanded population of cells. |