DNA CONSTRUCT AND METHOD FOR TRANSGENE EXPRESSION

摘要

This invention relates to a DNA construct that is capable of expressing a desired transgene in a trackable manner. The construct comprises in 5′ to 3′ downstream direction: a promoter; a fluorescent reporter gene positioned within an intron defined by a 5′-donor splice site comprising a splice donor sequence and a 3′-acceptor splice site comprising a splice acceptor sequence; a desired gene and a transcription terminator. A method of producing a transgenic host cell having a desired product is also disclosed.

主权项

1. A recombinant DNA construct comprising in order from 5′ to 3′:
(a) a promoter; (b) a fluorescent reporter gene positioned within an intron defined by a 5′-donor splice site comprising a splice donor sequence and a 3′-acceptor splice site comprising a splice acceptor sequence; (c) a desired gene; (d) a transcription terminator; wherein the promoter regulates transcription of both the fluorescent reporter gene and the desired gene; wherein the transcription terminator is unique.