Heterologous intron within an immunoglobulin domain

摘要

The invention concerns the field of recombinant gene engineering. It concerns novel introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes with heterologous introns, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel intron sequences as heterologous introns.

主权项

1. A transcription unit or expression vector comprising a gene of interest encoding at least one domain of an immunoglobulin gene, comprising at least one heterologous intron located within an immunoglobulin exon, whereby the 5′ and 3′ ends of said exon are defined as occuring in a corresponding native immunoglobulin gene.