Culture vessel and culture method

摘要

A microspace structure for highly efficiently producing spheroids of uniform size and easily replacing and recovering culture medium is designed, and a culture vessel having the designed microspace structure is provided. A plurality of recesses (10) comprising a bottom part (11) and an opening part (12) are arranged in the culture vessel. The bottom part (11) has the shape of either a hemisphere or a truncated cone, and the opening part (12) is constituted by walls having a taper angle of from 1° to 20° and surrounding an area from the boundary with the bottom part (11) to the end part of the recess (10). In addition, the equivalent diameter at the boundary is from 50 μm to 2 mm, the depth from the bottom of the bottom part (11) to the end part is 0.6 to 3 times the equivalent diameter, the walls constituting the opening part (12) form a continuous surface with the bottom part (11), and the slope of the continuous surface changes at the boundary.