NUCLEIC ACID ELEMENT CANDIDATE MOLECULE AND SCREENING METHOD FOR SCREENING FOR NUCLEIC ACID ELEMENT FOR TARGET ANALYSIS USING THE SAME

摘要

The present invention provides a novel nucleic acid element candidate molecule for use in screening for a nucleic acid element for target analysis and a novel screening method for screening for a nucleic acid element for target analysis using the same. The candidate molecule according to the present invention is a molecule for screening for a nucleic acid element for target analysis, being the following single-stranded nucleic acid (I): (I) a single-stranded nucleic acid comprising a catalyst sequence (D), a blocking sequence (B), and a binding sequence (A) that binds to a target, linked in this order. The blocking sequence (B) is complementary to a partial region (Dp) in the catalyst sequence (D). A terminal region (Ab) on the blocking sequence (B) side in the binding sequence (A) is complementary to a flanking region (Df) of the partial region (Dp) in the catalyst sequence (D) and is complementary to a terminal region (Af) on the side opposite to the blocking sequence (B) side in the binding sequence (A).

主权项

1. A nucleic acid element candidate molecule for use in screening for a nucleic acid element for target analysis, being either one of single-stranded nucleic acids (I) and (II):
(I) a single-stranded nucleic acid comprising:
a catalyst nucleic acid sequence (D) that exhibits a catalyst function;a blocking nucleic acid sequence (B); anda binding nucleic acid sequence (A) that binds to a target, linked in this order, whereinthe blocking nucleic acid sequence (B) is complementary to a partial region (Dp) in the catalyst nucleic acid sequence (D), anda terminal region (Ab) on the blocking nucleic acid sequence (B) side in the binding nucleic acid sequence (A) is complementary to a flanking region (DO of the partial region (Dp) in the catalyst nucleic acid sequence (D) and is complementary to a terminal region (Af) on the side opposite to the blocking nucleic acid sequence (B) side in the binding nucleic acid sequence (A); and (II) a single-stranded nucleic acid comprising:
a catalyst nucleic acid sequence (D) that exhibits a catalyst function;a blocking nucleic acid sequence (B);a binding nucleic acid sequence (A) that binds to a target; anda stabilization nucleic acid sequence (S), linked in this order, whereinthe blocking nucleic acid sequence (B) is complementary to a partial region (Dp) of the catalyst nucleic acid sequence (D), anda terminal region (Ba) on the binding nucleic acid sequence (A) side of the blocking nucleic acid sequence (B) is complementary to the stabilization nucleic acid sequence (S).