METHOD FOR PRODUCING DHA THROUGH SOLID CULTURE AND LIQUID FERMENTATION OF SCHIZOCHYTRIUM

摘要

The invention relates to a method for fermentation production of DHA, in particular a method for producing DHA through solid culture and liquid fermentation of Schizochytrium. The method comprises steps of activation of a strain, preparation of a suspension of the strain, preparation of a primary seed, preparation of a secondary solid seed, enlarging cultivation of the secondary solid seed in a fermentor, collection of cells after fermentation, and extraction of DHA oil. With the fermentation method, the seed preparation method is improved, the seed enlarging technology is simplified, the period of seed culture and fermentation is shortened, and therefore the investment is reduced and the cost is saved; in addition, the solid culture medium is of eutrophy and rich in a plurality of growth factors, and is more beneficial to the growth of the seed; cells are strong in viability and good in synchronism in solid culture medium.

主权项

1. A method for producing DHA through solid culture and liquid fermentation of Schizochytrium, which comprises the following steps:
(1) activating a strain, wherein a Schizochytrium strain is inoculated into a culture medium, cultured and activated to prepare a suspension of the strain; (2) preparing a primary seed, wherein the suspension of the strain obtained in step (1) is inoculated into a solid culture medium and cultured to obtain a primary solid seed; or wherein the suspension of the strain obtained in step (1) is inoculated into a liquid seed culture medium and cultured to obtain a primary liquid seed; (3) preparing a secondary solid seed, wherein the primary solid seed is prepared as a solution of the primary solid seed, which is inoculated into a solid culture medium and cultured; or wherein the primary liquid seed solution is inoculated into a solid culture medium and cultured; whereby a secondary solid seed is obtained; (4) enlarging culture of the secondary solid seed in a fermentor, wherein the secondary solid seed is prepared as a solution of the secondary solid seed, which is inoculated into a liquid culture medium in a fermentor for enlarging culture; and (5) collecting cells after fermentation and extraction of DHA.