| 摘要 |
The present invention has an object of providing a method for measuring the degree of polymerization in a protein using a very small sample amount, this method being simple and fast, and able to measure the degree of polymerization in a protein in a form which is closer to its in vivo structure than is permitted by conventional methods. The present invention is a method for measuring the degree of polymerization in a protein comprising the steps of: synthesizing a protein so that one molecule of subunit is labeled with one molecule of a fluorescent dye for all of subunits included in the protein; dissociating the protein to at least the subunit level; measuring the fluorescence intensity of the fluorescent dye within a small area before and after the dissociation; and determining the degree of polymerization in the protein based on the information relating to the fluorescence intensity obtained from the measurement.
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