| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for the selection of human cells for the production of human proteins by endogenous gene activation in order to produce human proteins in economical yields and in a form which is suitable for the production of a pharmaceutical preparation.SOLUTION: A method for the selection of human cell lines for the preparation of human proteins by the activation of a target gene endogenously present in a cell is characterized in that (a) a human cell line is tested for the presence of the following features: (i) the target gene with a desired nucleic acid sequence, (ii) at least 5 population doublings of the cell number within 14 days in a suspension culture, and (iii) at least 5 population doublings of the cell number within 14 days in a serum-free culture medium, and (b) using a cell line having features (i), (ii), and (iii) as starting cell line for the endogenous activation of the target gene. |
