DETECTION OF SHIGA TOXIN GENES IN BACTERIA

摘要

The disclosed invention is related to methods, compositions and kits for targeting nucleic acid derived from Shiga toxin-producing bacteria such as E. coli. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one pair of amplification oligomers.

主权项

1. A method for identifying a stx1 gene in a sample, said method comprising:
a) contacting said sample with a pair of amplification oligomers, each member of said pair of amplification oligomers having a length of from about 15 to about 25 contiguous nucleotides, said pair of amplification oligomers comprising an oligomer pair selected from the group consisting of
(i) a first oligomer having at least 90% sequence identity to SEQ ID NO:30 and a second oligomer having at least 90% sequence identity to SEQ ID NO:31;(ii) a first oligomer having at least 90% sequence identity to SEQ ID NO:1 and a second oligomer having at least 90% sequence identity to SEQ ID NO:2;(iii) a first oligomer having at least 90% sequence identity to SEQ ID NO: 8 and a second oligomer having at least 90% sequence identity to SEQ ID NO: 9;(iv) a first oligomer having at least 90% sequence identify to SEQ ID NO:12 and a second oligomer having at least 90% sequence identity to SEQ ID NO:13; and(v) a first oligomer having at least 90% sequence identity to SEQ ID NO:19 and a second oligomer having at least 90% sequence identity to SEQ ID NO: 20; b) amplifying nucleic acid in said sample with said pair of amplification oligomers to obtain an amplification product; and c) determining the sequence of said amplification product or detecting said amplification product using a detection probe.