Microscope and controlling method

摘要

A microscope includes a light source, a condenser lens disposed on an optical path of the light source to illuminate a specimen, an objective lens disposed on the optical path on an opposite side of the specimen from the condenser lens, a first polarizing plate disposed between the light source and the condenser lens, a compensator disposed between the condenser lens and the first polarizing plate to adjust retardation of light transmitted through the first polarizing plate, a second polarizing plate for transmitting only one-directional polarization component of the light transmitted through the specimen according to a relative positional relationship with the first polarizing plate, a driving unit for changing retardation of the compensator, and a control unit for causing the driving unit to drive the compensator to increase or decrease the retardation within a range including a position where the retardation is zero as a reference.

主权项

1. A microscope comprising:
a light source configured to generate light to illuminate a specimen; a plurality of optical units, each of which is disposable on an optical path of the light and is configured to change optical characteristics of the light, wherein an arrangement of each of the plurality of optical units on the optical path is configured to be changed according to each of a plurality of observation methods used for intracytoplasmic sperm injection; a condenser turret which has a disc shape and has a plurality of openings in a circumferential direction of a main surface of the condenser turret, each of the plurality of openings being configured to hold an optical element, and the condenser turret being configured to be rotated about a specified axis as a rotation axis so that one of the plurality of openings is disposed on the optical path; and a revolver which has a plurality of holding portions in a circumferential direction of a main surface of the revolver, each of the plurality of holding portions being configured to hold an objective lens, and one of the plurality of holding portions being configured to be disposed on the optical path, wherein, in the condenser turret, a plurality of relief contrast microscopy aperture plates are configured to be held in corresponding openings from among the plurality of openings, and magnifications of the plurality of relief contrast microscopy aperture plates gradually increase in the circumferential direction of the main surface of the condenser turret with respect to a specified empty opening which holds no optical element from among the plurality of openings, and wherein, in the revolver, a plurality of relief contrast microscopy objective lenses are configured to be held in corresponding holding portions from among the plurality of holding portions, and magnifications of the plurality of relief contrast microscopy objective lenses gradually increase in the circumferential direction of the main surface of the revolver with respect to a first objective lens having a minimum magnification and being held in one of the plurality of holding portions.