发明名称 |
METHODS FOR TARGETED MODIFICATION OF GENOMIC DNA |
摘要 |
Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more nucleic acid constructs fused with a pore-forming toxin, which may further be targeted to a particular cell type, wherein the nucleic acid constructs comprise an exogenous nucleic acid to be integrated into a genomic target site, and an enzyme, such as a nuclease or recombinase or a combination thereof capable of targeted introduction of the nucleic acid into the genome. |
申请公布号 |
WO2015188094(A1) |
申请公布日期 |
2015.12.10 |
申请号 |
WO2015US34471 |
申请日期 |
2015.06.05 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE;MASSACHUSETTS INSTITUTE OF TECHNOLOGY;COLLIER, R. JOHN;MCCLUSKEY, ANDREW J. |
发明人 |
COLLIER, R. JOHN;MCCLUSKEY, ANDREW J.;MARSISCHKY, GERALD;PENTELUTE, BRADLEY L. |
分类号 |
C12P21/04;C07H21/04;C07K14/00;C12N9/96;C12P19/34;C12Q1/34 |
主分类号 |
C12P21/04 |
代理机构 |
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代理人 |
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地址 |
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