| 摘要 |
Disclosed is an isolated antibody-producing cell that is a progeny of a non-human vertebrate cell whose genome comprises an immunoglobulin heavy chain locus comprising human gene segment JH6*02, one or more human VH gene segments and one or more human D gene segments upstream of a constant region; wherein the JH6*02 gene segment comprises the following nucleotide sequence: ATTACTA CTACTACTAC GGTATGGACG TCTGGGGCCA AGGGACCACG GTCACCGTCT CCTCAG, wherein the antibody-producing cell produces an antibody heavy chain specific for a target antigen which is an antigen of an infectious disease pathogen, and wherein the variable domain of the heavy chain is the product of recombination between a human VH, human D and human JH6*02 and wherein the HCDR3 length is at least 20 amino acids. Further disclosed are antibodies which bind an infectious disease pathogen which comprise said JH6*02 segment and have a HCDR3 of at least 20 amino acids and methods of making said antibodies. |
