NOISE REDUCTION METHODS FOR NUCLEIC ACID AND MACROMOLECULE SEQUENCING

摘要

Methods, systems, and devices are disclosed for processing macromolecule sequencing data with substantial noise reduction. In one aspect, a method for reducing noise in a sequential measurement of a macromolecule comprising serial subunits includes cross-correlating multiple measured signals of a physical property of subunits of interest of the macromolecule, the multiple measured signals including the time data associated with the measurement of the signal, to remove or at least reduce signal noise that is not in the same frequency and in phase with the systematic signal contribution of the measured signals.

主权项

1. A method for processing serial sequencing data, comprising:
obtaining a sequencing data set of a macromolecule having a plurality of subunits, wherein the obtained sequencing data set includes (i) signal data including multiple measured signals of a physical property of at least some of the subunits of the macromolecule and (ii) time data associated with a corresponding measured signal; determining a signal value for a particular subunit by cross-correlating the multiple measured signals including the time data associated with the corresponding measured signal to remove or at least reduce signal noise that is not in the same frequency and in phase with the systematic signal contribution of the measured signals; repeating the determining the signal value for another subunit of the macromolecule; and generating a data set of the determined signal values.