Lyase enzymes, nucleic acids encoding them and methods for making and using them

摘要

This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

主权项

1. A method for making modified small molecules comprising the following steps:
(a) providing a plurality of biosynthetic enzymes capable of synthesizing or modifying the small molecules, wherein one of the enzymes is an ammonia lyase enzyme encoded by a nucleic acid having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete sequence identity to SEQ ID NO:31; (b) providing a substrate of small molecules comprising at least phenylalanine, tyrosine and/or histidine; and (c) reacting the substrate of step (b) with the enzymes under conditions that facilitate a plurality of biocatalytic reactions to generate modified small molecules by a series of biocatalytic reactions.