| 摘要 |
The present invention relates to the field of cell and tissue culture. In particular, the invention provides a novel device for organotypic culture. T he device comprises a medium conduit having one open end and one end closed by a porous membrane fused across it; and a frame holding the medium conduit in a substantially vertical orientation; wherein the medium conduit is adapted to permit retention by capillarity of a sufficient volume of liquid culture medium in the medium conduit to contact the surface of the porous membrane a nd thus supply nutrients to cells that may be grown on the porous membrane. The device may further comprise a cell suspension conduit having one end open an d one end closed by the surface of said porous membrane contralateral to the surface of said porous membrane sealed to said medium conduit; wherein said frame holds the medium conduit and the cell suspension conduit in a substantially vertical orientation.
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