Expression vector system regulated byσ32 and methods for using it to produce recombinant protein

摘要

This invention discloses an expression vector system comprising a promoter recognized and regulated by a heat shock sigma factor of E. coli, especially &sgr;32. Preferably, the promoter comprises the consensus sequence of E. coli heat shock promoters as shown in SEQ ID NO:1. Also disclosed are methods for producing proteins using the promoter under heat shock conditions. Furthermore, the present invention discloses a method for creating a sudden temperature shift in a cell culture which has been pre-cultured to reach an optimal condition and which temperature shift will allow optimal production of a recombinant protein under the control a heat shock promoter.