IMPROVED BACTERIAL OUTER MEMBRANE VESICLE

摘要

PROBLEM TO BE SOLVED: To provide a further improved vesicle preparation together with a production process thereof.SOLUTION: A typical process includes the following steps of: (a) treating bacterial cells in the substantial absence of a surfactant; (b) centrifuging the composition from the step (a) to separate the outer membrane vesicles from the treated cells and cell debris, and collecting the supernatant; (c) performing a high-speed centrifugation of the supernatant from the step (b) and collecting the outer membrane vesicles in a pellet; (d) re-dispersing the pellet from the step (c) in a buffer; (e) performing a second high-speed centrifugation in accordance with the step (c) and collecting the outer membrane vesicles in the pellet; and (f) re-dispersing the pellet from the step (e) in an aqueous medium.