| 发明名称 |
METHOD OF QUANTIFYING PEPTIDE-DERIVATIVE LIBRARIES USING PHAGE DISPLAY |
| 摘要 |
The present application provides a method of quantifying an amount of a derivatized peptide displayed on a phage by phage display, the method comprising: providing a phage containing the target peptide thereon; reacting the phage containing the target peptide with a first reagent to derivatize the target peptide to form a derivatized peptide, reacting the derivatized peptide with a capture agent comprising a detection marker, thereby incorporating the detection marker within the derivatized peptide; and determining an amount of the detection marker, thereby quantifying the amount of the derivatized peptide displayed on the phage. A kit comprising a capture agent comprising a detection marker for quantifying the phage displayed derivatized peptides is also provided. |
| 申请公布号 |
US2015024958(A1) |
申请公布日期 |
2015.01.22 |
| 申请号 |
US201314375939 |
申请日期 |
2013.02.01 |
| 申请人 |
The Governors of the University of Alberta |
发明人 |
Derda Ratmir;Ng Simon;Jafari Seyed Mohammadreza |
| 分类号 |
G01N33/53 |
主分类号 |
G01N33/53 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of quantifying an amount of a derivatized peptide displayed on a phage by phage display, the method comprising:
providing a phage containing a target peptide displayed thereon; reacting the phage containing the target peptide with a first reagent to derivatize the peptide to form a derivatized peptide, reacting the derivatized peptide with a capture agent comprising a detection marker, thereby incorporating the detection marker within the derivatized peptide; and determining an amount of the detection marker, thereby quantifying the amount of the derivatized peptide displayed on the phage. |
| 地址 |
Edmonton CA |
