Compositions and methods for diagnosis of genetic susceptibility, resistance, or tolerance to infection by mycobacteria and bovine paratuberculosis using promoter variants of EDN2

摘要

Provided are methods for determining susceptibility, resistance or tolerance to Mycobacterium avium subspecies paratuberculosis (Map) infection, comprising: determining, using a biological sample from a mammalian subject, a presence or absence of at least one EDN2 gene mutation, or the genotype of at least one EDN2 gene single nucleotide polymorphism (SNP) selected from the SNP group consisting of SNP105 (nucleotide position 105,288,174 on BTA3 (UMD 3.1)), SNP208 (nucleotide position 105,298,664 on BTA3 (UMD 3.1)), and SNP272 (nucleotide position 105,305,070 by on BTA3 (UMD 3.1)), that segregates with resistance and/or tolerance to Map tissue infection; and determining, based thereon, at least one of susceptibility, resistance or tolerance of the mammalian subject to Map infection. Additionally provided are mammals (e.g., bovine) derived culling, selecting, or selective breeding, based on disclosed methods, and methods for procuring mammals. Preferably, the mammals carry non-susceptible alleles of SNP105 (G), SNP208 (G), and SNP272 (G).

主权项

1. A method for culling, selecting, or breeding a mammalian subject, comprising:
determining, using a biological sample from the subject, a presence or absence of at least one EDN2 gene mutation, or the genotype of at least one EDN2 gene single nucleotide polymorphism (SNP) selected from the SNP group consisting of SNP105 (nucleotide position 105,288,174 on BTA3 (UMD 3.1)), SNP208 (nucleotide position 105,298,664 on BTA3 (UMD 3.1)), and SNP272 (nucleotide position 105,305,070 by on BTA3 (UMD 3.1)), that segregates with resistance and/or tolerance to Map tissue infection, wherein said step of determining a presence or absence of at least one EDN2 gene mutation comprises
extracting DNA from said biological sample,contacting said DNA to a probe immobilized on a solid support, anddetecting the presence or absence of a DNA-probe complex; determining, based thereon, at least one of susceptibility, resistance or tolerance of the subject to Mycobacterium avium subspecies paratuberculosis (Map) infection; and at least one of culling, selecting, or breeding the subject, based on the determining of at least one of susceptibility, resistance or tolerance of the subject to Map infection.