发明名称 |
célula bacteriana gram-negativa recombinante, e, método para produzir uma proteína recombinante |
摘要 |
A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes:
a. a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene;
b. a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and
c. a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity ;
wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated Deg P gene and optionally a polynucleotide sequence encoding a protein of interest. |
申请公布号 |
BR112012006670(A2) |
申请公布日期 |
2015.09.08 |
申请号 |
BR20121106670 |
申请日期 |
2010.09.23 |
申请人 |
UCB PHARMA S.A |
发明人 |
DAVID PAUL HUMPREYS;MARK ELLIS |
分类号 |
C12N9/50;C12P21/00 |
主分类号 |
C12N9/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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