Method for the preparation of diols

摘要

The present invention concerns a new method for the biological preparation of a diol comprising culturing a microorganism genetically modified for the bioproduction of an aliphatic diol, wherein the microorganism comprises a metabolic pathway for the decarboxylation of a hydroxy-2-keto-aliphatic acid metabolite with an enzyme having a 2-keto acid decarboxylase activity, the product obtained from said decarboxylation step being further reduced into the corresponding aliphatic diol, and wherein the microorganism is genetically modified for the improved production of said hydroxy-2-keto-aliphatic acid metabolite.;The invention also concerns a modified microorganism for the production of an aliphatic diol.

主权项

1. An E. coli microorganism genetically modified for the bioproduction of an aliphatic diol, wherein
the microorganism comprises a metabolic pathway for the decarboxylation of a hydroxy-2-keto-aliphatic acid metabolite with an enzyme having a 2-keto acid decarboxylase activity encoded by the gene kivD from L. lactis, the product obtained from said decarboxylation step being further reduced into the corresponding aliphatic diol with an enzyme having hydroxyl aldehyde reductase activity encoded by the gene yqhD from E. coli, and the microorganism is genetically modified for the improved production of said hydroxy-2-keto-aliphatic acid metabolite by an increase of the homoserine transaminase activity or the homoserine oxidase activity encoded by SerC from E. coli.