| 摘要 |
PROBLEM TO BE SOLVED: To provide novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that the polysaccharides may be covalently linked (conjugated) to proteins either directly or through a spacer.SOLUTION: These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1-CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The conjugated proteins are used for manufacture of vaccines. |
