Infectious DNA vaccines against chikungunya virus

摘要

Described herein are i-DNA™ vectors and vaccines and methods for using the same. The i-DNA™ generates live attenuated vaccines in eukaryotic cells in vitro or in vivo for pathogenic RNA viruses, particularly chikungunya virus (CHIKV). When iDNA is injected into the vaccine recipient, RNA of live attenuated virus is generated by in vivo transcription in the recipient's tissues. This initiates production of progeny attenuated viruses in the tissues of the vaccine recipient, as well as elicitation of an effective immune response protecting against wild-type, non-attenuated virus.

主权项

1. A vector comprising:
(a) DNA encoding an infectious RNA molecule; and (b) a eukaryotic RNA polymerase promoter;wherein:
(i) the DNA encoding an infectious RNA molecule is operably linked to the eukaryotic RNA polymerase promoter; and (ii) the infectious RNA molecule encodes a chikungunya virus (CHIKV).