ENGINEERED ANTIBODY FRAGMENTS FOR TARGETING AND IMAGING CD8 EXPRESSION IN VIVO

摘要

Disclosed herein, the parental antibodies from the hybridomas YTS 169.4.2.1 (YTS169) and 2.43 were engineered into minibody and diabody fragments. Both the YTS and 2.43 antibodies bind mCD8+. However, they differ in that the YTS 169 antibodies bind both Lyt2.1 and Lyt2.2 while the 2.43 antibodies bind an epitope that is Lyt2.2 specific. These novel minibodies and diabodies retained their antigen specificity as shown by flow cytometry and ImmunoPET imaging. Most importantly, both the 2.43 and YTS169 minibodies and diabodies produced high contrast ImmunoPET images of CD8+ lymphoid organs at only four hours post-injection.

主权项

1. An antibody that binds to CD8 comprising:
The VH domain of any of SEQ ID NOs.: 7, 15, or 23, and The VL domain of any of SEQ ID NOs.: 11, 19, or 27.