COMPOSITIONS AND METHODS FOR IMPROVING NANOPORE SEQUENCING

摘要

The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.

主权项

1. A method of analyzing a polymer, comprising:
applying an electric field across a nanopore disposed between a first conductive liquid medium and a second conductive liquid medium in to establish an interaction between the nanopore and a polymer in the first conductive medium, and measuring an ion current to provide a current pattern, wherein the polymer comprises an analyte domain and an end domain, wherein the end domain has a first charged moiety, and wherein a difference in the ion current from the threshold amount in the current pattern indicates a characteristic of the polymer.