发明名称 |
A METHOD OF PREPARING A GROWTH FACTOR CONCENTRATE DERIVED FROM HUMAN PLATELETS |
摘要 |
The invention relates to a method of preparing an intra-dermally, intra-articularly, sub-dermally or topically administrable growth factor concentrate derived from human platelets. The method comprises the steps of suspending human platelets in multiple electrolyte isotonic solution; snap-freezing the suspension; thawing the frozen suspension; and sterile-filtering the suspension. In particular, in this method, a fixed number of platelets is suspended in a fixed volume of multiple electrolyte isotonic solution to obtain the required concentration of growth factors in the growth factor concentrate, snap-freezing of the suspension is carried out at a temperature of −120° C. to −200° C., thawing of the frozen suspension is carried out at 25° C. to 37° C., and cellular debris are separated from the thawed suspension and the resultant suspension of growth factors is diluted with an isotonic medium before sterile-filtering. |
申请公布号 |
EP2884992(A1) |
申请公布日期 |
2015.06.24 |
申请号 |
EP20130879335 |
申请日期 |
2013.04.22 |
申请人 |
KASIAK RESEARCH PVT. LTD. |
发明人 |
TOTEY, SATISH MAHADEORAO;MANIYAR, RACHANA RAJIV;FONSECA, LYLE CARL;LAGHATE, SNEHA DEEPAK;KOSHY, NICOLE |
分类号 |
A61K38/18;A61K38/22;C12N15/09 |
主分类号 |
A61K38/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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