Heat-stable respiratory syncytial virus F protein oligomers and their use in immunological compositions

摘要

Heat-stable oligomeric recombinant polypeptides, presenting at least one antigenic epitope of the pre-fusion Respiratory Syncytial Virus (RSV) F protein, comprising the RSV F protein ectodomain, functionally deleted in the HRB region, transmembrane and cytoplasmic domains replaced with a heterologous trimerization domain, and absent two functional multibasic furin cleavage sites, are useful as antigenic components in immunogenic compositions useful in methods of inducing an immune response and vaccinate against RSV infections.

主权项

1. A heat-stable oligomeric recombinant polypeptide presenting at least one antigenic epitope of the pre-fusion Respiratory Syncytial Virus F protein, said polypeptide comprising
the Respiratory Syncytial Virus F protein ectodomain, from which the HRB region is deleted, and from which the transmembrane and cytoplasmic domains are deleted and replaced with a heterologous trimerization domain, and wherein the two multibasic furin cleavage sites in said Respiratory Syncytial Virus F protein ectodomain are mutated by the substitution of all lysine residues in said sites with arginine residues, thereby rendering said furin cleavage sites defective.