Method for detecting single nucleotide polymorphism in nucleic acid

摘要

A method for detecting a single nucleotide polymorphism in nucleic acids, characterized in that the method includes mixing (A) a nucleic acid probe comprising a nucleotide sequence complementarily hybridizable to an evaluation subject nucleic acid/an antisense strand thereof containing at least one single nucleotide polymorphism, and tagged with a nucleotide sequence of a hairpin structure having a bulge at a 5′-terminal thereof, wherein a guanine residue is introduced at an adjoining position of the bulge, and wherein a naphthyridine derivative compound is immobilized to the bulge; and (B) the evaluation subject nucleic acids; and detecting a signal ascribed to the naphthyridine derivative compound when the above nucleic acid probe and the above evaluation subject nucleic acids are hybridized, thereby evaluating the above single nucleotide polymorphism.

主权项

1. A method for detecting a single nucleotide polymorphism in nucleic acids, characterized in that the method comprises:
mixing (A): i) a nucleic acid probe comprising a nucleotide sequence complementarily hybridizable to an evaluation subject nucleic acid comprising at least one single nucleotide polymorphism, and tagged with a nucleotide sequence of a hairpin structure having a cytosine bulge or thymine bulge at a 5′-terminal thereof, wherein a guanine residue is introduced at a position immediately adjoining 5′ or 3′-terminal side of the cytosine bulge or thymine bulge, and wherein a 2,7-diaminonaphthyridine derivative compound is immobilized to the cytosine bulge or thymine bulge; or ii) a nucleic acid probe comprising a nucleotide sequence complementarily hybridizable to an antisense strand of an evaluation subject nucleic acid comprising at least one single nucleotide polymorphism, and tagged with a nucleotide sequence of a hairpin structure having a cytosine bulge or thymine bulge at a 5′-terminal thereof, wherein a guanine residue is introduced at a position immediately adjoining 5′ or 3′-terminal side of the cytosine bulge or thymine bulge, and wherein a 2,7-diaminonaphthyridine derivative compound is immobilized to the cytosine bulge or thymine bulge; and (B) the evaluation subject nucleic acids; and detecting a signal ascribed to the 2,7-diaminonaphthyridine derivative compound due to the disappearance of the above cytosine bulge or thymine bulge when said nucleic acid probe and said evaluation subject nucleic acids are hybridized, thereby evaluating said single nucleotide polymorphism.