Mice that make binding proteins comprising vl domains

摘要

Disclosed is a mouse, comprising in its germline an immunoglobulin hybrid chain locus comprising at least one unrearranged light chain variable region (VL) gene segment, at least one unrearranged light chain joining (JL) gene segment, and an immunoglobulin heavy chain constant region nucleic acid sequence capable of associating with a light chain constant region, wherein each of the unrearranged VL and JL gene segments comprise recombination signal sequences that allow the unrearranged VL and JL gene segments to recombine such that the mouse further comprises a rearranged immunoglobulin hybrid gene comprising light chain variable region (VL/JL) nucleotide sequence operably linked with the immunoglobulin heavy chain constant region nucleic acid sequence.