鳖蛋胜肽之制备方法、其产物及其用于制备降血压药物之用途;A METHOD FOR MANUFACTURING A PEPTIDE OF PELODISCUS SINENSIS, THE PEPTIDE OF PELODISCUS SINENSIS, AND THE USE OF THE PEPTIDE OF PELODISCUS SINENSIS BEING MANUFACTURING A DRUG FOR REDUCING BLOOD PRESSURE THEREOF

摘要

一种鳖蛋胜肽制备方法，系以简单的步骤获得具有降低血管增压素转换酶活性之鳖蛋胜肽，其包含：提供一鳖蛋蛋白溶液，系将一鳖蛋蛋白溶于一缓冲液；提供一嗜热菌蛋白酶，该嗜热菌蛋白酶活性单位为50~100U/mg；以酵素受质比为1/50~1/900之重量比例混合该嗜热菌蛋白酶及该鳖蛋蛋白，于酸硷值为pH 6~9及温度50~70℃之条件中进行水解反应1~10小时，以得到一水解溶液，该水解溶液系含有鳖蛋胜肽，且该鳖蛋胜肽系包含如SED ID NO：1所示序列。 A method with simple steps for manufacturing a peptide ofPelodiscus sinensiswith activity of inhibiting angiotensin I-converting enzyme is disclosed. The method comprises: providing an egg white solution dissolved with a buffer, providing thermolysin with activity being 50-100 U/mg, and mixing the thermolysin with the egg white in a E/S weight ratio of 1/50-1/900, followed by hydrolysis the egg white solution at pH 6-9, 40-60℃