| 主权项 |
1. A method for preparing an allicin solution comprising the following steps:
(A) extracting allicin, which comprises the following steps: (1) adding alliinase and deoxygenated pure water to a container, in a water bath, filled with argon or high purity nitrogen in a water-to-alliinase ratio of 1000-2000 ml to 35.4 g, stirring thoroughly to completely dissolve the alliinase, adjusting the temperature of the water bath to 25-35° C., and controlling pH at 6.5-8.5 to obtain an alliinase solution; (2) preparing an alliin solution in a water-to-alliin ratio of 500-1000 ml to 35.4 g, adding the alliin solution dropwise into the container containing the alliinase solution, stirring thoroughly, and continuously filling the container with argon or high purity nitrogen so that the container is fully filled with the argon or high purity nitrogen, wherein a ratio of alliin to alliinase is 1 mg to 1-2 IU; and (3) after completion of the dropwise addition of the alliin solution, continuously stirring and reacting for 20 to 40 minutes in total to obtain an enzymolysis reaction solution containing allicin, immediately replacing warm water in the water bath with ice water to lower the temperature of the water bath to 0° C., and using an ultrafiltration membrane of 1000-5000 Da to remove alliinase to obtain an aqueous solution of allicin; and (B) diluting the aqueous solution of allicin with a solvent precooled to a temperature of 0-4° C. within a clean bio-safety cabinet to obtain an allicin solution. |
