发明名称 |
Native wharton's jelly stem cells and their purification |
摘要 |
Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided. |
申请公布号 |
US9012222(B2) |
申请公布日期 |
2015.04.21 |
申请号 |
US201313752928 |
申请日期 |
2013.01.29 |
申请人 |
Auxocell Laboratories, Inc. |
发明人 |
Taghizadeh Rouzbeh R. |
分类号 |
C12N5/00;C12N5/02;C12N5/073;C12N5/07;A61L27/38 |
主分类号 |
C12N5/00 |
代理机构 |
Goodwin Procter LLP |
代理人 |
Goodwin Procter LLP |
主权项 |
1. A method of purifying noncultured Wharton's Jelly stem cells and culturing mesenchymal stem cells, the method comprising:
(a) digesting an umbilical cord tissue comprising Wharton's Jelly, wherein the digesting comprises mincing the umbilical cord tissue; (b) subsequently diluting the umbilical cord tissue; (c) subsequently filtering the umbilical cord tissue to generate a filtrate comprising digested Wharton's Jelly and the noncultured Wharton's Jelly stem cells, wherein undigested umbilical cord tissue is filtered from the filtrate; (d) sedimenting the noncultured Wharton's Jelly stem cells from the filtrate generated in step (c), thereby separating the noncultured Wharton's Jelly stem cells from the digested Wharton's Jelly; (e) storing the noncultured Wharton's Jelly stem cells at a temperature below −20° C.; and (f) culturing mesenchymal stem cells from undigested umbilical cord tissue, wherein the mesenchymal stem cells are cultured in the digested Wharton's Jelly. |
地址 |
Cambridge MA US |