Modified glucose dehydrogenase

摘要

The purpose of the present invention is to provide an FAD-conjugated glucose dehydrogenase that is hard to be inhibited by the inhibitors such as 1,10-phenanthroline.;The present invention relates to a modified glucose dehydrogenase (GLD), comprising an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1 having a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 298, 338, 340, 341, 343, 352, 354, 424, 426, 431 and 432, wherein the modified GLD has a reduced susceptibility to an inhibitor, as compared with the wild-type GLD, especially to said modified GLD, which has 40% or more of a relative activity when determined in a system wherein the inhibitor coexists at a final concentration of 1 mM based on an enzymatic activity when determined in a system wherein the inhibitor does not coexist.

主权项

1. An isolated polypeptide having glucose dehydrogenase (GLD) activity, wherein the polypeptide comprises all of SEQ ID NO: 1 except for at least one substitution at a position selected from the group consisting of positions 298, 338, 340, 341, 343, 352, 354, 426, 431 and 432 of SEQ ID NO: 1, and wherein the polypeptide has reduced susceptibility to inhibition by 1,10-phenanthroline, as compared with the polypeptide of SEQ ID NO: 1.