FLUORESCENCE MICROSCOPES WITH POLYCHROIC MIRROR CHANGERS

摘要

Fluorescence microscopy systems with polychroic mirror changers are described. In one aspect, a polychroic mirror changer includes a polychroic-mirror array. The array includes a plate with a planar surface and a number of holes formed in the plate. The array also includes polychroic mirrors attached to the planar surface of the plate such that each polychroic mirror covers one of the holes and the reflective surface of each polychroic mirror is adjacent to and aligned with the planar surface of the plate. Each polychroic mirror is partially exposed through a corresponding hole to reflect a different subset of excitation channels of a beam of excitation light input to the changer. The polychroic-mirror array can be mounted in the changer so that when a different subset of excitation channels is selected to illuminate a specimen, the plate is moved within a single plane of motion.