INTRODUCING SAMPLES INTO SUPERCRITICAL FLUID CHROMATOGRAPHY SYSTEMS

摘要

A supercritical fluid chromatography system is provided with an injection valve subsystem for introducing a sample into a flow of mobile phase fluid. The injection valve subsystem includes an auxiliary valve and an inject valve. The operations of the auxiliary and inject valves are coordinated in such a manner as to reduce sample carry-over and system pressure perturbations occurring during sample injection.

主权项

1. A method comprising
pressurizing a flow path connected to a separation column with a mobile phase fluid comprising liquefied CO2; then pressurizing fluidic tubing and a sample loop containing a sample slug with the mobile phase fluid; and then establishing fluidic communication between the fluidic tubing and the separation column and thereby introducing the sample slug into the pressurized flow path.