POLYACRYLAMIDE GEL FOR USE WITH TRADITIONAL AND NON-TRADITIONAL ELECTROPHORESIS RUNNING BUFFERS

摘要

Disclosed are gel systems prepared with a substantially neutral gel buffer solution, which contains an amine base and at least one zwitterionic component and an acid component. Methods of making and using these gel systems are also disclosed herein.

主权项

1. A method of preparing a gel system, the method comprising: polymerizing acrylamide in the presence of a cross-linking agent and a gel buffer solution, wherein the gel buffer solution comprises an amine base at a concentration of from about 0.005 to about 0.25 M and a zwitterionic component at a concentration of from about 0.005 to about 0.5 M titrated with an acid component that comprises a polyprotic acid to a pH of from about 6.0 to about 9.5.