CEREBROSPINAL FLUID ASSAY

摘要

The present invention is directed to methods of isolating particles, such as nucleic acid-containing particles or microvesicles, from a biological sample and extracting nucleic acids therefrom, wherein the biological sample is cerebrospinal fluid. The present invention further provides methods for aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker associated with a disease or medical condition thereof.

主权项

1. A method of extracting nucleic acids from a biological sample, comprising the steps of:
a. isolating nucleic acid-containing particles from the biological sample by one or more centrifugation procedures, wherein none of the centrifugation procedures are performed at a speed exceeding about 200,000 g; b. performing one or more steps to mitigate adverse factors that prevent or might prevent high quality nucleic acid extraction; and c. extracting nucleic acids from the isolated particles.