发明名称 |
ENZYME CONCENTRATION AND ASSAYS |
摘要 |
Methods of conducting an assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity are provided. The methods include concentrating the glycoprotein having enzymatic activity by capturing the glycoproteins from the input sample on a solid support, washing the glycoproteins captured on a solid support to remove unbound portions of the input sample, adding an enzyme substrate to the output sample comprising glycoproteins having enzymatic activity, and measuring enzymatic modification of the substrate. In some examples, the capturing of the glycoprotein and subsequent washing steps are performed in one or more droplets in oil. |
申请公布号 |
EP2707724(A4) |
申请公布日期 |
2015.01.21 |
申请号 |
EP20120781874 |
申请日期 |
2012.05.09 |
申请人 |
ADVANCED LIQUID LOGIC, INC. |
发明人 |
GRAHAM, CARRIE;ECKHARDT, ALAN;PERKINS, LISA |
分类号 |
G01N33/68;C12N9/24;G01N1/28;G01N33/53 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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