IMMUNOGENIC POTE PEPTIDES AND METHODS OF USE

摘要

POTE has recently been identified as a tumor antigen expressed in a variety of human cancers, including colon, ovarian, breast, prostate, lung and pancreatic cancer. Described herein are immunogenic POTE polypeptides, including modified POTE polypeptides, that bind MHC class I molecules. The immunogenic POTE polypeptides are capable of inducing an immune response against POTE-expressing tumor cells. Thus, provided herein is a method of eliciting an immune response in a subject, such as a subject having a type of cancer that expresses POTE.

主权项

1. An isolated nucleic acid molecule encoding an immunogenic polypeptide, wherein the polypeptide comprises no more than 10 consecutive amino acids of the amino acid sequence of human POTE set forth as:
MVAEVCSMPTASTVKKPFDLRSKMGKWCHHRFPCCRGSGKSNMGTSG DHDDSFMKMLRSKMGKCCRHCFPCCRGSGTSNVGTSGDHENSFMKML RSKMGKWCCHCFPCCRGSGKSNVGAWGDYDHSAFMEPRYHIRREDLD KLHRAAWWGKVPRKDLIVMLRDTDMNKRDKEKRTALHLASANGNSE VVQLLLDRRCQLNVLDNKKRTALIKAIQCQEDECVLMLLEHGADRNIPD EYGNTALHYAIYNEDX1LX2AKAX3LX4YGADIESKNKCGLTPLLLGVHE QKQQVVKFLIKKKANLNVLDRYGRTALILAVCCGSASIVNX5LX6EQNX7DVSSQDLSGQTAREYAVSSHHHVICELLSDYKEKQMLKISSENSNPEQD LKLTSEEESQRLKVSENSQPEKMSQEPEINKDCDREVEEEIKKHGSNPVG LPENLTNGASAGNGDDGLIPQRRSRKPENQQFPDTENEEYHSDEQNDTR KQLSEEQNTGISQDEILTNKQKQIEVAEQKMNSELSLSHKKEEDLLRENS VLQEEIAMLRLELDETKHQNQLRENX8X9X10EEIX11SVKEKTDKLLRAM QLNEEALTKTNI (SEQ ID NO: 20), wherein X1 is K or Y; X2 is M, A or F; X3 is L or A; X4 is L or V; X5 is L or Y; X6 is L, A or F; X7 is V, or A; X8 is K or Y; X9 is I or L; X10 is L or F; and X11 is E or A; and wherein the polypeptide comprises one of (a) amino acids 323-331; (b) amino acids 222-230; (c) amino acids 252-260; (d) amino acids 272-280; or (e) amino acids 553-561 of SEQ ID NO: 20, wherein the polypeptide does not comprise the amino acid sequence KLMAKALLL (SEQ ID NO: 3).