SMALL CONDITIONAL RNAs

摘要

The present subject matter relates to the use conditional hairpins, such as, but not limited to shRNAs. The conditional formation of these structures can allow for further events, such as gene silencing (in some embodiments).

主权项

1. A method of conditional Dicer substrate formation, the method comprising:
providing a duplex that comprises a first strand hybridized to a second strand; and combining the duplex to a mixture for detection of a target strand, wherein a presence of a target strand results in a displacement of the first strand from the second strand, and wherein the displacement of the first strand from the second strand allows for the second strand to form an shRNA, and wherein the shRNA is formed in a conditional manner.