| 主权项 |
1. A method for quantifying skin changes caused by heat evil, the method comprising the steps of:
(a) selecting a skin stimulus (i.e., heat or heat temperature) for the heat evil from temperature ranging from 39° C. to 50° C.; (b) selecting a suitable cellular biochemical method, which can measure skin cell changes, according to the selected skin stimulus, wherein the suitable cellular biochemical method is selected from the group consisting of TNF-α ELISA, PGE2 ELISA, interleukin expression analysis, cell proliferation assay, FAS analysis, RT-PCR, immunocytochemistry, melanin analysis, catalase assay, dichlorofluorescin diacetate assay (DCFH-DA), DAPI staining, β-gal staining, MTT assay, LDH assay, comet assay, and Western blot analysis employing specific antibodies; (c) measuring condition of the skin of a subject by the selected cellular biochemical method; (d) stimulating the skin with the selected skin stimulus, and then quantifying the degree of cellular changes according to the intensity of the selected heat temperature; and (e) applying an extract of Lonicera japonica to the skin, and then measuring and quantifying the conditions of the skin by the selected cellular biochemical method. |
