Methods for identifying and isolating cells expressing a polypeptide

摘要

Disclosed is a polypeptide comprising: (i) an extracellular portion that comprises an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains and does not comprise an immunoglobulin heavy chain variable region; and (ii) a non-immunoglobulin transmembrane portion. The polypeptide comprises a sequence as defined in the complete specification. Also disclosed is a method of identifying a cell that produces a heterodimeric molecule of interest comprising: (a) transfecting a cell that expresses a first polypeptide with a polynucleotide that encodes at least one second polypeptide with the first polypeptide comprising (i) an extracellular portion that comprises an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains and does not comprise an immunoglobulin heavy chain variable region; and (ii) a nonimmunoglobulin transmembrane portion with the second polypeptide comprises an immunoglobulin heavy chain; and wherein a heterodimeric molecule comprising the first polypeptide and the second polypeptide is expressed on the surface of the cell; (b) contacting the cell with a detection molecule, wherein the detection molecule binds the heterodimeric molecule; and (c) identifying the cell bound by the detection molecule with the method not being performed within a human being.