发明名称 |
FORBEDRET EKSPRESSIONSFREMGANGSMÅDE |
摘要 |
The present invention concerns a method of producing a desired heterologous gene product wherein said heterologous gene product is expressed from a strong promoter, said method comprising expressing said gene using a mutant mRNA leader which comprises one or more mutations which enhance transcription of the gene. The invention also provides a mutant Pm mRNA leader sequence, and a vector and a library comprising the leader sequence. Methods of obtaining an mRNA mutant leader and identifying an mRNA mutant leader are encompassed, along with a vector for selection or identification of an mRNA leader mutant and a use thereof for screening. |
申请公布号 |
DK2059599(T3) |
申请公布日期 |
2014.11.17 |
申请号 |
DK20070766423T |
申请日期 |
2007.08.03 |
申请人 |
VECTRON BIOSOLUTIONS AS |
发明人 |
VALLA, SVEIN;BAKKE, INGRID;LALE, RAHMI;BERG, LAILA |
分类号 |
C12N15/70;C12N15/09 |
主分类号 |
C12N15/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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