Method for post-extracting high-acyl gellan gum

摘要

Disclosed is a method for extracting high acyl gellan from the fermentation broth containing gellan gum with a low production cost and high quality of products, comprising the following steps: (1) Treatment of the fermentation broth with an enzyme; (2) flocculation of the treated fermentation broth with acid; (3) wash of the fiber-like material; and (4) drying and milling.

主权项

1. A post extraction process for preparing high acyl gellan, comprising the following steps:
(1) Adding an enzyme preparation into a fermentation broth to conduct a reaction by raising the temperature of the broth; (2) Cooling down the fermentation broth treated by the enzyme in step (1), adding an acid to flocculate the fermentation broth, and then conducting a solid-liquid separation; (3) Washing the solid fiber-like material obtained from the solid-liquid separation in step (2) first with a low polar or non-polar solvent, then conducting a solid-liquid separation, and then washing the solid fiber-like material with a lower alcohol, and then conducting a solid-liquid separation; and (4) Drying and disintegrating the solid material obtained in step (3), to obtain the high acyl gellan product.