Immunodisplacement electrophoresis

摘要

The disclosure teaches a method for the analysis of a sample by electrophoresis, making use of a binding partner for a target compound or group of target compounds which may be present in the sample. The disclosure further teaches a kit for use in an electrophoretic analysis, to a modified antibody or fragment thereof, and to specific uses of the kit or modified antibody or fragment thereof.

主权项

1. A method for the analysis of a sample comprising one or more proteins, wherein the sample is selected from the group consisting of blood serum and urine, the method comprising the steps of:
a. separating a first portion of the sample by electrophoresis into constituent parts; b. mixing a second portion of the sample with a binding partner for a target compound or a group of target compounds, wherein the target compound or the group of the target compounds comprise an immunoglobulin, and the binding partner is a macromolecule comprising:
(i) a binding segment capable of specifically binding to the target compound or the group of the target compounds, the binding segment selected from the group consisting of antibodies and fragments thereof; and(ii) a polyanionic poly(amino acid);wherein the binding segment and the polyanionic poly(amino acid) are covalently linked;wherein the mixing of the binding partner and the target compound or the group of the target compounds produces a complex; and c. separating the second portion by electrophoresis into constituent parts, wherein a protein or proteins of the sample that have not produced a complex with the binding partner are separated from the complex of the binding partner with the target compound or the group of the target compounds in a separation medium in which the binding partner has a net negative charge and wherein a non-complexed binding partner and the complex of the binding partner with the target compound or the group of the target compounds are migrated out of protein bands; d. comparing the separated constituent parts of step (a) with the separated constituent parts of step (c).