发明名称 |
Method for detecting replication or colonization of a biological therapeutic |
摘要 |
Methods for detecting replication in or colonization of a host by a biological therapeutic, such as an oncolytic virus, cells administered for cell therapy and gene therapy vectors, are provided. In the methods, a product produced by the biological therapeutic is detected in a sample of tissue or body fluid distinct from the administered therapy or locus thereof, thereby permitting assessment of the therapy and/or monitoring its progress. |
申请公布号 |
US8859256(B2) |
申请公布日期 |
2014.10.14 |
申请号 |
US201213573845 |
申请日期 |
2012.10.05 |
申请人 |
Genelux Corporation |
发明人 |
Szalay Aladar A.;Stritzker Jochen;Hess Michael |
分类号 |
C12N15/52;C12N15/86;G01N33/569;G01N21/64;C12Q1/68 |
主分类号 |
C12N15/52 |
代理机构 |
McKenna Long & Aldridge LLP |
代理人 |
McKenna Long & Aldridge LLP ;Seidman Stephanie |
主权项 |
1. A method for treating cancer and monitoring therapeutic progress in a subject having tumors, said method comprising:
a) administering to said subject an oncolytic virus encoding a β-glucuronidase, wherein said oncolytic virus targets and treats cancer and the β-glucuronidase activity indicates oncolytic viral colonization or replication in said cancer; b) obtaining a sample from a subject, wherein said sample is a body fluid or tissue sample that is not a tumor sample; c) detecting β-glucuronidase activity in the sample, wherein said detection is by the addition of a substrate for β-glucuronidase; and d) determining the presence of a product catalyzed by the reaction of the β-glucuronidase with the substrate, wherein the detection of the product indicates that the oncolytic virus has colonized or is replicating in a tumor tissue or cell in the subject and is treating said tumor tissue or cell and oncolytic viral therapy be continued. |
地址 |
San Diego CA US |