发明名称 |
METHOD FOR PREPARING SMOOTH MUSCLE CELL CULTURE |
摘要 |
FIELD: medicine.SUBSTANCE: invention relates to biotechnology, cell technologies and tissue surgery. A method for preparing a smooth muscle cell culture consists in cutting a blood vessel fragment, grinding it to a piece size of no more than 2 mm in any dimension, and incubating the pieces in a culture flask having its bottom preliminarily scratched and containing a culture medium containing 10% embyo foetal serum for at least ten days, but no more than 24 days at 37°C in the COincubator environment; the method differs by the fact that the above blood vessel fragment is an ascending thoracic aorta fragment cut during the coronary artery bypass surgery; before the incubation, the above pieces of the ascending thoracic aorta fragment are kept in the culture medium containing 0.1% collagenase for at least 30 minutes, but no more than 60 minutes at 37°C and then washed in the cell culture medium.EFFECT: invention enables preparing the cells of the aortic tissue directly from the patient's vital tissues for transplantology applications.3 dwg |
申请公布号 |
RU2529947(C2) |
申请公布日期 |
2014.10.10 |
申请号 |
RU20120152110 |
申请日期 |
2012.12.05 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE "FEDERAL'NYJ TSENTR SERDTSA, KROVI I EHNDOKRINOLOGII IMENI V.A. ALMAZOVA" MINISTERSTVA ZDRAVOOKHRANENIJA ROSSIJSKOJ FEDERATSII |
发明人 |
MALASHICHEVA ANNA BORISOVNA;KOSTAREVA ANNA ALEKSANDROVNA;ZVEREV DMITRIJ ANATOL'EVICH;KOSTINA DAR'JA ALEKSEEVNA;USPENSKIJ VLADIMIR EVGEN'EVICH;GORDEEV MIKHAIL LEONIDOVICH;URUSOVA MARIJA EVGEN'EVNA |
分类号 |
C12N5/071;C12N5/02;C12N5/077 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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