Protein complex having activity catalyzing asymmetric oxidation reaction and process for producing the same

摘要

A process for producing a cross-linked crystallized protein complex, which comprises: a first step of concentrating a crude protein derived from an animal or plant; a second step of encapsulating the protein in a gel, to thereby allow the protein to undergo air oxidation, and then extracting a protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation; and a fourth step of cross-linking the precipitated protein complex. Alternatively, by use of a fifth step of drying (FD) the obtained crosslinked crystallized protein complex, to thereby form a powder. As a result, there is provided an enzyme which is stable at room temperature storage, and has an activity in catalyzing an asymmetric oxidation reaction. That is, there is provided a useful material which enables an efficient enzyme-mimetic reaction under a mild condition.

主权项

1. A process for producing a cross-linked crystallized protein complex, comprising:
a first step of concentrating a crude protein from a water-soluble moiety, the crude protein obtained from animal or plant resources; a second step comprising:
encapsulating the protein in a gel;oxidizing the gel encapsulated protein by leaving the gel to stand in the air, wherein the oxidation forms a protein complex; and
extracting the protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation in an aqueous solution; and a fourth step of cross-linking the precipitated protein complex to form a cross-linked crystallized protein complex.