摘要 |
The present invention relates to a method for high production of recombinant bifunctional acetyl xylan esterase with a carbohydrate binding module derived from phanerochaete chrysosporium. Acetyl xylan esterase produced from yeast which is transformed to an acetyl xylan esterase gene with a carbohydrate binding module(CBM) derived from phanerochaete chrysosporium not only has an activity of removing an acetyl group of a xylan backbone structure and decomposing xylan and lignin, but also improves an activity of decomposing biomass when used with xylanase at the same time, thereby being used as an industrial enzyme for feed additives, production of paper, a detergent, decomposition of lingo-cellulose-based biomass, and production of bio-energy. |