POLYNUCLEOTIDES AND POLYPEPTIDES INVOLVED IN POST-TRANSCRIPTIONAL GENE SILENCING

摘要

This invention relates to isolated nucleic acid fragments encoding polypeptides involved in post-transcriptional gene silencing. The invention also relates to construction of a recombinant DNA construct encoding all or a portion of the polypeptide involved in post-transcriptional gene silencing, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels in a transformed host cell of the polypeptide involved in post-transcriptional gene silencing.

主权项

1. A recombinant DNA construct comprising a polynucleotide operably linked to at least one heterologous regulatory sequence, said polynucleotide comprising:
(a) a nucleotide sequence encoding a polypeptide, wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 8, or (b) the complement of the nucleotide sequence of (a),wherein a transgenic plant expressing the polynucleotide has an altered level of transposon silencing compared to a non-transgenic plant.